Video - Lecture 5 (Part1/2) of 1st International Deuterium Symposium


Videos DAF 75 Lecture 5 (Part1/2) of 1st International Deuterium Symposium



Lecture 5 (Part1/2) of 1st International Deuterium Symposium

Protective effects of DDW in Caenorhabditis Elegans model About the lecture: 1) Avila DS, 2) Somlyai G, 1) Aschner M 1) Vanderbilt Medical Center, Nashville TN, USA 2) HYD LLC for Cancer Research and Drug Development, Budapest, Hungary The nematode Caenorhabditis elegans is a unique model organism for studies on longevity and the aging process. The worm is an attractive system because of its size (1mm in lenght), short life span (~ 20 days) and its homology to the human genome (60-80%). The knowledgebase obtained through genetic analyses strongly suggests that endocrine signalling plays a key role in many of the pathways that alter the aging process in C. elegans, including insulin/IGF-1-like signalling. In order to study the putative anti-aging effects of depleted deuterium water (DDW), we used a well-standardized manganese (Mn) model of toxicity in C.elegans, in which it has been demonstrated that Mn causes lethal osmoregulation defects, developmental delay, dopaminergic neurodegeneration and decrease in lifespan. In the present study we analyzed the ability of DDW to attenuate the Mn-induced effects. A synchronous population of N2 (wild type) worms was treated with 35 mM MnCl2 (4000 worms per tube in) for 30 minutes and then washed 3 times to remove residual Mn. Next, worms were treated for 48 hrs with a D-concentration of 150 ppm (M9 buffer in regular water), 120 ppm (M9 buffer prepared with DDW in a ratio 25: 75 of M9 in regular water) or 90 ppm DDW (M9 buffer ...

Anti, Aging, DDW, Cancer, Krebs, manganese, C.elegans

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Duration: 9 minutes : 59 seconds
Author: DDWInfos
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